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Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
In a manner not dissimilar to the theory of chaos, it seems reasonable to suppose that a casual smile in Battersea can lead to an marriage in Bolton and a spontaneous street party in Tunbridge Wells (well, if a theory isn't far-fetched, it's unworthy of the name).
Table 1 Aquifer parameters evaluated in dug wells Well no.
Table 1 Total and faecal coliform results for studied wells Well Total coliforms Av.
Table 2 Different indices of specific capacity calculated for dug wells Well no.
Forty observation wells, well distributed around the two water harvesting structures were monitored fortnightly for the below-groundwater levels.
Similar(36)
The crestal wells (Well-3 and Well-1) in Area A have similar rock characteristics as seen in Fig. 15.
The field started producing through the wells Well-1 and Well-2 from February 2000 and December 2000, respectively.
Subsequently, the other wells (Well-3 to Well-8) were drilled and put on production in different years until 2009.
Two new wells (Well-7 and Well-8) were drilled in 2009, and their locations are marked in Fig. 12.
The case studies carried out here consider six oil-producing wells (Well-1 to Well-6) from the total of 8 oil-producing wells.
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