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Cells in each well were provided with 30 µl loading reagent (6x, Protocol for Beta-lactamase loading solutions, Invitrogen) and 150 µl CO2-independent medium supplemented with 10% serum and IL-2.
Ramos cells (2 4×106 cells per well) were provided to obtain a 2∶1 target: trogocyte ratio and were allowed to settle onto RAW cells for 10 minutes at 4°C.
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Warranties are often a different matter, though, because they may well be provided by a specialist provider that will still honour them.
Greek fountains were utilitarian as well, being provided with ample draw basins and reservoir supply and often shaded by a portico.
Last, the history of well is provided.
In addition, a design procedure for hydraulic fractures in a gas well is provided.
This trusted entity, which is referred to as the Access Control Provider (ACP), may as well be provided by the enterprise itself, for example, by leveraging its user management system, or by a third party.
Nevertheless, this impulse for the anatomy course might well be provided by other departments or faculties.
Health education should also be adjusted to the patient's own individual needs and could well be provided by diabetes nurses [ 43].
Wells were provided with fresh media and transfection mix prepared in 0.5 ml Optimem was added with gentle mixing.
That said, the one thing Prototype does especially well is provide an enjoyable sort of locomotion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com