Sentence examples for well were maintained in from inspiring English sources

HUVECs (1 × 10 cells per well) were maintained in 24-well plates until 60 70% confluence.

Briefly, cells (1 × 10 cells per well) were maintained in 100  μl medium containing 0.5% FBS for 48 h in the presence or absence of 6  μM cyclopamine.

The cultures were run in duplicates and after 48 hrs of culture, cells were collected for total RNA extraction while cells in the remaining wells were maintained in culture until supernatants were collected at day 9 and stored at −20°C until tested.

Negative control wells were maintained in DMEM supplemented with 10% CS for the duration of the assay.

As a negative control an equal number of wells were maintained in expansion media for 20 days.

Cells were seeded in 6-well plates (BD Biosciences, Bedford, MA, USA) at a density of 1 × 10 cells per well, and were maintained in complete medium.

The seeded scaffolds, placed in 6 well dishes, were maintained in a humidified atmosphere at 37 °C and 5% CO2 in an incubator.

Clear 24-well plate were maintained in parallel to assess confluence.

Briefly, cells (1×10 cells/well in 96-well plates) were maintained in OBM at 37 °C for 24 h.

At the completion of the illumination period, 10-μl aliquots were removed from the illuminated and non-illuminated wells (control cells were maintained in 96-well plates covered with aluminum foil at room temperature for the duration of the illumination) and serially diluted tenfold in PBS to generate dilutions of 10−1 to 10−4 times the original concentrations.

HUVEC cells (8×10 cells/well in the filter of a 48-well transwell plate) were maintained in 10% FBS (v/v) for 48 h.