Sentence examples for well we added from inspiring English sources
For each well, we added 200 μl PBS and 20 μl CytoX-Violet (indicator solution) and incubated the well plates at 37 °C for 120 min in darkness; 190 μl of the indicator solution from each well was transferred to sterile black 96-well plates, and fluorescence intensities were recorded.
To each well we added 100 µL of 5 µM Aβ toxic oligomers and inhibitors at various concentrations.
To calculate the number of phosphate ions present in the well, we added the concentration of phosphate ions used (0.7 mM) to the concentration of phosphate already present in DMEM (0.9 mM).
To each well, we added 75 μL RBC suspension and mixed gently by pipetting.
Authors: Well, we added a statement as follows "The true difficulty of intubtion according to the standard of direct laryngoscopy remains unknown".
In each well we added the mouse anti- Lgals1 antibody (2 μg/ml, R&D Systems Minneapolis, MN) and mouse anti-beta-tubulin antibody (2 μg/ml, Chemicon, Temecula CA) and the slides incubated in a humid chamber overnight at 4°C.
In sample wells, we added 25 μL of assay buffer and 25 μL of culture supernatant to make a final volume of 50 μL.
The supernants containing pyrene-actin were mixed with the lysate, added into 96-well plate and held for three min. In the control wells, we added either 1μM jaspakinolide, an actin polymerization agent, or 0.5 μg/ml latrunculin B, an actin depolymerization agent.
When the leaves were well chopped, we added an additional 0.5 mL of cold chopping buffer.
When the JAR cell cultures reached 50%to80%0% confluence in 6-well plates, we added NB (2 MCF), nHAP (2 MCF), or PBS (control) to the wells (200 μL/well) for 48 h.
