After the tagmentation reaction, indexing specific PCR primers were added two per well for unique dual indexing of the libraries for multiplex sequencing.
The number of overlapping segments between marker types was determined as well as those segments unique for a marker.
On the other hand, IGF-1R has been shown to be crucial for anchorage independent growth, a property being well established to be unique for malignant cells.
Adaptor 1*: 5′AATGATACGGCGACCACCGAGATCTACACGCCTCCCTCGCGCCATCAG-3′; Primer 1*: 5′-AATGATACGGCGACCACCGA-3′; Primer 2*: 5′-CAAGCAGAAGACGGCATACGA-3′; Adaptor 2: 5′-CAAGCAGAAGACGGCATACGAGAT-[BAR CODE]*-CGGTCTGCCTTGCCAGCCCGCTCAG-3′ Adapter 2 carried a 7 bp barcode sequence unique for each well of two 96-well plates (Supplementary file 2I).
Located in Houston, Texas, our National School has created one of the very first tropical medicine clinics for indigent patients affected by these diseases (headed by Dr. Laila Woc-Colburn), as well as unique training for physicians and medical students to recognize, diagnose and treat these infections (with a unique teaching laboratory headed by Dr. Kathryn Jones).
Surface based sensor platforms have benefited from miniaturization of the capture area and particle capture systems provide a route to further surface miniaturization, as well as unique opportunities for the rapid analysis of dilute samples of particular interest for point-of-care (POC) diagnostics.
The site contains an advanced, searchable repository of Tibetan objects, as well as unique features for creating and sharing descriptive annotations about the objects and their significant parts, allowing for a collaborative decoding and description of Tibetan culture itself.
As part of BBC Scotland's Memorandum of Understanding between the two organisations, we offer a mentoring Scheme to young orchestral players, a regular opportunity for upcoming soloists to get a chance to perform with the orchestra, as well as unique projects for composers and conductors.
Thus there is both functional overlap as well as unique roles for these two different STAT3 spliceforms.
Furthermore, there are also peptides that are unique for plasma as well as for CSF and brain tissue (Additional file 1: Table S1).
