Four Hybaid Ribolyser beads were placed into each well of a 96-well transparent flat-bottom plate; then 200 µl reaction mixture containing diluted protein and ThT was pipetted into wells, and the plates were covered using an optically corrected adhesive film.
A parallel set of cells (5 × 10 cells/well in a 24-well transparent plate) were assayed for intracellular fluorescence using a fluorescence microscope (OLYMPUS CKX 41), with images captured at the magnification of 20×.
Ownership requires community participation as well as transparent and accountable decisions by government.
Today's EAC report is right to suggest any new system should be mandatory and fit for purpose from the outset, as well as transparent so it commands the respect of all involved, including the public.
Public trust and public participation in research demand clear stewardship as well as transparent and accountable oversight.
(a) The cut surface of the resected specimen showed a multilocular cystic lesion with many papillary nodules projecting from the lining wall, as well as transparent mucinous fluid.
However, Amazon still has bragging rights thanks to its wins in prior years for "Mozart" and its star Gael Garcia Bernal, as well as "Transparent" and its lead, Jeffrey Tambor.
Critical discourse analysis (CDA) aims at analysing the 'opaque as well as transparent structural relationships of dominance, discrimination, power and control as manifested in language' (Wodak 1995, p. 448).
