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In announcing his candidacy in South Carolina on Saturday, he pointed to his policies of low taxes, reduced government spending and regulatory easing as "a recipe to produce the strongest economy in the nation" and one that Washington would do well to duplicate.
For induction of RNAi, cells were re-suspended in Schneider's media at a density of 4 × 105 cells/ml and 75 μl of cell suspension was added per well to duplicate 96-well cell culture plates that had been spotted with 5 μl of dsRNA per well.
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Briefly, seven days after transfection, supernatant from each transfected well of the 6-well plate was transferred to duplicate wells of a 12-well plate seeded with VERO cells.
If all goes well, TMP hopes to duplicate the service in up to 100 markets, starting at year-end.
A quantity of 100 μl of serum-free medium, TLR agonist or IFN-α dilution, or water dilution, was added to duplicate wells of a 96-well tissue culture plate (BD Biosciences, San Jose, CA, USA).
Briefly, samples were rapidly thawed from –70°C, and 200 μL of each 10-fold dilution (10-1 10-6) of serum in MEM was added to duplicate wells of a six-well plate seeded with 4-day-old Vero MARU cells.
A quantity of 100 μl of either SFM, the TLR2 agonist dilution or the corresponding water dilution was added to duplicate wells of a 96-well plate along with 100 μl of PBMCs at a concentration of 5 × 105 cells/ml in SFM.
Samples were incubated with either 1 μL of a TF-blocking antibody (hTF1) (4 μg/mL) or 1 μL of a control antibody (mouse IgG: 4 μg/mL) for 15 minutes at 25°C and then 50 μL aliquots were added to duplicate wells of a 96-well plate.
The flexibility of using a traditional paper-based medium to present information offers many possibilities for users to adapt such forms relatively easily using word processing and graphical software packages, as well as the ability to duplicate them on demand using a variety of print media.
In the case of VDAC, the evolving structure function paradigm necessitates that the encoding gene redundancy should indicate a need for transport function innovation as well as the tendency to duplicate the genetic material as suggested for invertebrates, plants, and vertebrates (Saccone et al. 2003).
Dilutions (1 20 to 1 60) of plasma samples were then added to duplicate wells; 1% BSA in PBS was added to 'blank' wells in quadruplicate.
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