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Following this incubation, unbound cells were washed off by rinsing each well three times with PBS (137 mM NaCl, 3 mM KCl, 8 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4).
The percentage of cell viability, as blue/total cells, was assayed by scoring 200 cells per well three times.
The detached cells were removed by gently washing the well three times with 100 mM PBS (pH 7.4) at 37°C.
After washing each well three times with water, the fluorescence of each well was analyzed using a Spectramax M5 plate reader (Molecular Devices, Sunnyvale, CA) with excitation 630 nm, emission 697 nm, and cutoff 695 nm, background corrected by subtracting the mean signal from empty wells and normalized to the mean value of untreated wells.
Cup water in your hands and rinse your mouth well three times.
Similar(55)
(Well, nine times out of ten).
On their second drive, the Falcons ran the ball well: six times for 31 yards.
"He'll do the same this week and what he usually plans, well, nine times out of 10 it works.
Mr Keerthisinghe tells of one Sri Lankan farmer who drained a contaminated well five times after the tsunami had receded, only to find seawater seeping back.
She goes to the well five times a day for her extended family household of 62 people.
After scratching, gently wash the well two times with a medium to remove the detached cells, and the well was replenished with a fresh medium.
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