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This property is well supplemented with previous results (Shmelev, A.N., Kulikov, G.G., 1997. About neutron-physical features of the modified (denatured) fuel cycles.
Then totally 9,216 colonies from both PB2 N-terminal and C-terminal truncation libraries were isolated using a colony-picking robot (KBiosystems, Basildon, UK) into 384 well plates containing 70 µl TB medium per well (supplemented with kanamycin and chloramphenicol).
They were allowed to attach for 3 h, after which 150 μl of DMEM was added to each well, supplemented with 2% FBS.
Colonies grown on LB-kanamycin (60 μg/ml) agar plates were transferred, by using toothpicks, into 96-well microtiter plates containing 150 μl of MDG non-inducing medium [ 41] in each well supplemented with kanamycin (60 μg/ml).
Slices were then incubated in the coated plates containing 1.3 ml Williams Medium E glutamax-I (Gibco, Paisley, UK) per well, supplemented with 25 mM D-glucose and 50 µg/ml gentamycin.
We diluted liquid cultures of wild-type cells (in stationary phase) 1000-fold into 96 individual 150 μL cultures on a single microplate, with each well supplemented with 1.0 μg/mL tetracycline.
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A proportion of the PKH26+ target cells (<20%) was positive for 7-AAD; however, this was not significantly different between control wells containing A02 cells alone and coculture wells supplemented with vehicle alone or ABT-737 (data not shown).
Briefly, in the upper well of the invasion chamber, 5 × 10 cells were seeded per well in DMEM without serum; in the lower chamber well, DMEM supplemented with 10% FBS were added to stimulate the cell invasion.
Twenty droplets of cell suspension (total volume ~ 5 µL) were dispensed into each well of a 96-well plate supplemented with 100 µL of fresh culture medium (Fig. 3a).
A 700-μl volume of each well was supplemented with 700 μl neutralizing solution as described by Tilton (Tilton and Rosenberg 1978) (1.0 g sodium thioglycolate + 1.46 g sodium thiosulfate in 1000 ml deionized water).
A 700-μl volume of each well was supplemented with 700 μl neutralizing solution as described by Tilton and Rosenberg (1978) (1.0 g sodium thioglycolate + 1.46 g sodium thiosulfate in 1.000 ml deionized water).
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