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Consistent with previous studies [ 33- 35], we found that paedomorphic populations are primarily restricted to streams with chert gravel, which is very well sorted into large size classes.
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Single sperm were sorted into wells of 96-well V-bottom plates using a fluorescence activated cell sorter.
Briefly, single CD19+CD27- and CD19+CD27+ cells were sorted into wells of 96-well plates using a FACStar Plus flow cytometer with an automated single cell deposit unit (Beckton-Dickinson, USA).
Populations of 36 animals, each containing different percentages of adult worms were sorted into wells of a 384-well microtiter plate (Figure 1C).
Cells were then index sorted into wells of a 96-well plate and analyzed further.
Single cells were sorted into wells of a 96-well plate to generate clonal lines.
The widget itself seems fairly well designed, with games sorted into categories like "Sports" and "Puzzles".
Each single GFP-positive cell was sorted into one well of a 96-well plate by fluorescence-activated cell sorting (FACS) technology.
A single cell was sorted into each well of a 96-well ultra-low-attachment plate (Corning Life Sciences, Tewksbury, MA) containing 200 μL of stem cell medium per well.
CD184+/CD271−/CD44−/CD24+ NSC derived from NDC3.1 were sorted into 96 well plates at one cell per well on a feeder layer of mitotically inactivated mouse astrocytes to improve the survival of the single cells.
IL-10 and IFN-γ secreting Ves v 5 specific CD4+ T-cells were identified using the cytokine secretion assay and single cells were sorted into individual well in round-bottomed 96-well plates using a MoFlo cell sorter.
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