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The optimal well solution for crystallization contained 0.1 M MgCl 2, 0.5 M KCl, 16% polyethylene glycol (PEG) 3,350 (Sigma-Aldrich) and 0.05 M Hepes-NaOH buffer at pH 7.2.
For soaking experiments, crystals were transferred to a solution containing 10 mM MgCl2 or MnCl2, 22% (w/v) PEG 4000, 100 mM Tris pH 6.8, 20% (v/v) glycerol, and 1 mM Raltegravir and incubated over the well solution for 18 h.
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However, though these methods could enhance low illumination images at a certain extent, they might also lead to contrast over-enhancement or noise amplification (as shown in Fig. 2) due to constrains of stretching range in the solving model, or difficulty of designing of parameters, and well solutions for the problems are still opened.
The well solution used for growing the crystals contained 0.2 M ammonium sulphate, 0.1 M Tris pH 8.5 and 25% (w/v) PEG3350.
Crystals were soaked in a cryoprotectant solution (well solution complemented with 20 25% glycerol) for 1 5 min and then flash-cooled in liquid nitrogen.
Because of the numerical implementation simplicity and well captured solution for fluid dynamics around multiple bodies, Lattice Boltzmann Method (LBM) has been adopted in lots of domains during the last two decades.
Using a well-known solution for three-dimensional solute transport from a planar source, an approximate solution is derived for the maximum plume length at steady-state conditions.
We use as a benchmark the progressive edge growth (PEG) code design technique [20], which is a well-consolidated solution for designing very good unstructured LDPC codes.
Austria's chancellor, Wolfgang Schüssel, said the constitution was a well-balanced solution for an expanded union.
"We know from our many developments that increased quality and availability of owner-occupied retirement housing provides a well-deserved solution for our customers.
It is the most common and well-known solution for clustering [23].
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