Sentence examples for well solution from inspiring English sources

MccB co-crystals were cryo-protected in well solution plus 25% glycerol and flash frozen in liquid nitrogen for subsequent X-ray diffraction data collection.

Prior to crystallization, the protein was diluted with water to 4 mg ml−1 and mixed with a well solution at a 1 1 v/v ratio.

Diffraction-quality crystals were obtained by mixing 2 μl of protein solution with 2 μl of well solution (5 11% MPD, 0.2 M magnesium acetate and 0.1 M MES pH 6.5 6.8) using the hanging drop vapour diffusion method.

Crystals were harvested and protected in the well solution containing 30% (w/v) PEG3350 and cooled in dry nitrogen stream at 100 K for X-ray data collection.

After being soaked for a few seconds in cryoprotection solution (well solution complemented with 25% xylitol), the crystals were cooled by plunging them into liquid nitrogen.

The drop contained 1 µl of protein solution and 1 µl of well solution, and equilibration was carried out over 500 µl of well solution.

The crystal was washed three times in the well solution and put in SDS-buffer.

For the hanging drop method, the protein drop is suspended above the well solution from a seal and makes no contact with the crystallisation plate.

The crystals were soaked in the well solution plus 10 mM NaSO4 for 10 minutes before flash-frozen in liquid nitrogen.

Crystallization experiments with varying concentrations of CaCl2, SrCl2 or BaCl2 in the well solution instead of MgCl2 did not yield satisfactory crystals.

Crystallization experiments were also conducted with MnCl2, CaCl2, BaCl2 and SrCl2 in the well solution instead of MgCl2 but did not yield diffracting crystals.