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After 8 h, the cells of the first well were harvested, lysed and the total extracts were maintained at −20°C; 12 ng/ml of LMB were added to the second well, and the third well served as control; both the second and third wells were incubated for an additional 4 h at 37°C in 5% CO2.
The twelfth well served as control since no sample (extract, or reference antibiotics) was added in it.
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Triton X (0.1%), DMSO (0.1%), and untreated wells served as controls.
Untreated wells served as controls.
Cells in the remaining wells served as controls.
An uninoculated well served as a control.
The cells in the fourth well served as normal control group.
These wells serve as controls to estimate nonspecific binding levels of GNRs to cells.
Wells serving as controls for the effect on cytokine levels of in vitro cell culturing per se, received SFM containing NRC-1 gas vesicles.
In each 8-well strip, one BSA-coated well served as a negative control.
SV-Renilla luciferase plasmids of 5 ng per well served as the internal control.
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