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We utilized an inoculum of 100 cell culture 50% infectious doses (CCID50) per well seeded with 20 000 MDCK cells to form a monolayer in 96-well microtiter plates.
After 5 to 7 days, each well seeded with a wild-type cell was filled with rosettes, while wells seeded with mutant cells defective in rosette development were expected to produce cultures of solitary cells and/or chain colonies, but few to no rosettes, even in the presence of ACM.
siRNA treatments were carried out in 6-well plates, each well seeded with 1×10 hTERT-1604 cells or 1×10 NIH 3T3 cells, before reverse transfection using Dharmafect 1 (Thermo Fisher Scientific) and 80 nM (NIH 3T3) or 100 nM hTERT-16044) ON-TARGETplus SMARTpool DNMT1 siRNA or a matched concentration of scrambled control (Thermo Fisher Scientific).
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The wells seeded with MC3T3-E1 cells in the absence of scaffolds were treated as positive control, and the empty wells without cells were used as negative controls.
Unlabelled bevacizumab (0.01 to 1000 nM) and 1 nM of 111In-bevacizumab were added to wells seeded with cells (2 × 105) and incubated at 4 °C for 2 h.
MTT assay data for: ×0.1, ×1, and ×10 respectively of initial 100 μL of 10−9 M AgNP added to culture wells seeded with 1,000 cells/100 μL.
After five days in culture, wells seeded with 1, 2, 3 and >3 cells per microbubble well were inspected for cell proliferation.
Each PM plate contains 96 wells seeded with different metabolite and monitored automatically over time via the OmniLog machine.
Our simulation study consisted of 142 plates, each plate comprising 384 wells seeded with 306 drugs, 16 negative controls and 8 positive controls.
For subsequent analysis, in order to achieve statistical confidence, we transfected 12 to 30 wells seeded with primary cells, in seven different independent experiments.
Wells seeded with a wild-type cell (gray circles) produced a culture with abundant rosette colonies, while wells seeded with a rosette defect mutant (black circle) produced a culture with chains or single cells, but few to no chain colonies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com