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The main objectives of these tests were to determine how well SEED clusters the sequences with respect to the number of clusters, and the number of falsely assigned members in them compared with the true clusters.
To evaluate how well SEED clusters NGS data, we designed test experiments with real datasets where we benchmarked its performance against the 'true' clusters obtained from genome alignment results.
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Five thousand cells per well seeded on 96-well plates and incubated overnight at 37°C (5% CO2).
Cells (5 × 10 per well) seeded in 96-well plates were treated with required concentrations of the drug.
Seeds were direct sown in 64-well seeding trays and 14-day-old seedlings were transplanted into pots containing 6 kg of clay loam soil.
Women of this planet need some essential resources: wells, seeds and roads.
From a total of 6,720 wells seeded, 45 clonal populations were produced, 32 of which survived expansion to ∼106 cells.
Each PM plate contains 96 wells seeded with different metabolite and monitored automatically over time via the OmniLog machine.
For lentivirus infection, 1 × 10 cells in a well were seed into 12-well plates.
In the macrophage infection experiments, 5×104 cells per well were seeded in 96-well plate and 2.5×105 cells per well were seeded in 24-well plate.
HepG2 cells (5 × 104 per well) were seeded into 24-well plates.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com