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"Some people say the storage facilities are reasonably well replenished," he told the Guardian.
Hawthorne caught the sense of a nation in decline by describing Boston's repurposed Province House, in his day, as "a bar in modern style, well replenished with decanters, bottles, cigar boxes... and provided with a beer pump".
"Come into a store on Wednesday, and shelves will be fairly well replenished, things will be back in order, there will be lots of workers to give you attention and much less commotion," ShopperTrak founder Bill Martin said.
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Each drop was placed in the center of one well of an adherent 24-well plate and incubated for 15 min. After gel solidification, each well was replenished with 400 μl of MEBM medium supplemented with the same factors described in the oncosphere formation assay.
In this assay, cells were plated in 96-well plates, after which media containing drug was added to each well and replenished every 48 h.
After scratching, gently wash the well two times with a medium to remove the detached cells, and the well was replenished with a fresh medium.
When the cell cultures were confluent, the culture medium was removed from each well and replenished with 0.1 mL of the maintenance medium.
100 μL of the release media was removed from the inner well and replenished with fresh media at a series of time points (days 1, 3, 7, 11, 14, 18).
Culture supernatants were harvested every four days, and wells were replenished with fresh medium containing cytokine conditions that were identical to the medium used on day 1.
The wells were replenished with fresh medium supplemented with 20 ng/ml recombinant murine GM-CSF on days 2 and 4. The cells were harvested as indicated.
His scheme calls for adding flood walls, strategically placed levees and surge barriers as well as replenishing sand dunes and protecting and increasing natural barriers, such as wetlands.
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CEO of Professional Science Editing for Scientists @ prosciediting.com