The upper well content was removed using cotton swabs and the number of cells that migrated was estimated using an XTT cell proliferation kit (Biological Industries) according to manufacturer's instructions.
Chloride, sulphate, and carbonate, as well as metals can be removed using cation exchangers in the Ag-, Band and H-form [23].
Then, 300 μL of 0.2 % Triton X-100 was added to each well, and the cells were removed using a cell scraper (BD, Franklin Lakes, NJ) to extract the DNA.
This study aims to determine the type and magnitude of the residual stresses in the porcelain veneers of full-contour fixed-dental prostheses (FDPs) with an anatomic zirconia coping design and in control porcelain with the zirconia removed using a well-established Vickers indentation method.
Homopolymer tracts and adaptors were removed using perl scripts as well as by manual examination.
Fresh CT was removed using a sickle and dried in a dark, well-ventilated place.
After the desired incubation time, the liquid medium was removed using a vacuum and the planktonic cells were removed by washing the well once with MilliQ water.
They found that the pigs showed certain characteristic types of behaviour: they rooted in the soil, exercised their neck muscles by levering against fallen logs, built nests in secluded areas before giving birth, and used dunging areas well removed from their resting areas.
This technique affords many of the advantages of standard optical manipulation techniques (e.g., optical tweezers[28], plasmonic tweezers[29]), however using far less optical power (up to 105× less[27]) as well removing the requirement of static electrodes used for more conventional DEP manipulation platforms[16], [20], [30], [31].
