Sentence examples for well reconstituted in from inspiring English sources
The cysteine-rich M1 and M2 domains and their loop structures [ 54] contain conformational epitopes and are generally not well reconstituted in a prokaryotic expression system.
Here, we apply electron cryotomography (ECT) to image native chemoreceptor arrays as well as selected chemoreceptor complexes reconstituted in vitro.
Here we show images of cellular arrays as well as selected chemoreceptor complexes reconstituted in vitro that reveal new principles of array structure and assembly.
In this process, the full biosynthetic pathway for the production of a target compound is first identified and isolated from the native producer, and then reconstituted in a well-characterized and easily culturable heterologous producer like Escherichia coli.
Although light scattering tend to flatten out the CD spectra obtained from protein in lipid vesicles making a direct comparison to micelles difficult, the characteristic α-helical structure for aquaporins is rather well preserved when the SoPIP2 1 is reconstituted in lipid complex membranes of E. coli lipids (Figure 1 and Table 1).
(C ) Silver stained SDS-PAGE gel of recombinant partial and holo DKC1 as well as NAF1-containing intermediate complexes reconstituted in E. coli cells expressing various epitope-tagged subunits and untagged DKC1 as denoted.
To keep the boundary conditions comparable, the MinCDE oscillations and gradients in Figure 1 were reconstituted in tapered compartments as well.
Typically, 20 μL of 1X passive lysis buffer (reconstituted in water) was added to each well of the 96-well plate and subjected to brief agitation on an orbital shaker.
Finally, we illustrate the application of these methods to microcrystalline protein preparations as well as single and multi-span membrane proteins reconstituted in lipid membranes.
This protein has been purified, reconstituted in an artificial membrane system as well as structurally and functionally characterized [ 42, 43].
Radiolabeled bacteria were then reconstituted in PBS and 3% BSA, added to the well at 108 CFU/well, and incubated at room temperature for 2 h.
