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The cysteine-rich M1 and M2 domains and their loop structures [ 54] contain conformational epitopes and are generally not well reconstituted in a prokaryotic expression system.
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High titers of anti-Palm-SOC4-II-Sm4 antibodies were generated, which recognize the priming immunogenic conjugate, as well as reconstituted Sm mimics but not the carrier alone.
This paper describes the development of new test apparatus that can be used for soil specimens obtained from routine site investigation as well as reconstituted specimens.
Analyses of PST080 ES cells as well as reconstituted frog oocytes demonstrated that ΔCD98hc-β geo fusion protein preserved its ability to interact with integrin β1 although this mutant protein was hardly localized on the cell surface.
"We don't have some of the larger entities here today because I want to help them rethink their role in the city as well and reconstitute themselves," Garcetti told the group.
We now have identified Syk mutant that are altered in auto-inhibition and tested their activity in the S2 Schneider cell reconstitution system as well as in reconstituted Syk deficient B cell lines.
For Pyr3, we found that this compound rapidly suppresses both native CRAC currents as well as heterologously reconstituted CRAC currents upon acute administration (Supporting Information Fig. S1A,B).
In the framework of the following sections, many different modeling approaches will be presented and discussed in close conjunction with experimental data from whole-cell studies as well as from reconstituted systems.
The motile behaviour of mammalian dynein has been studied using complexes purified from brain (Mallik et al, 2005; Ross et al, 2006; Miura et al, 2010; Ori‐McKenney et al, 2010; Walter et al, 2010) and tissue culture cells (Ichikawa et al, 2011), as well as complexes reconstituted from individual, recombinant components (Trokter et al, 2012).
We tested this by integrating transgenes into reconstituted attP40 and reconstituted attP2, as well as into native attP40 and attP2.
But the popes kept control of the Campagna, and the belt of papal lands between Rome and Ravenna remained intact as well; the Papal States, as reconstituted by the late-medieval popes, reproduced almost exactly the boundaries of the former exarchate.
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