Sentence examples for well reactions from inspiring English sources

Following the Real-Time PCR reaction random target well reactions from each diet group were run on a 2% agarose gel to verify amplicon singularity and size.

Real-time PCR was performed in 12.5- μl (384 well) reactions with 2 ng of template DNA.

All reactions were performed using an Applied Biosystems 7900HT real-time PCR instrument equipped with a 384 well reaction plate.

Real-time PCR reactions (25 µl) were done in 96 well reaction plates adding 1× SYBR Green PCR Master Mix (Applied Biosystems), 5 µl cDNA and 400 nM primers.

These reactions were performed using 24 well reaction blocks and an automatic reagent-dispensing platform under inert atmosphere.

cDNA amount corresponding to 6.35 ng of the total RNA was used for a single well reaction, to achieve an average Ct of 23.

PCR was performed on the ABI Prism 7900 HT Fast Real-time PCR Sequence Detectinn System (applied Biosystems) in a 384 well reaction plate according to the manufacturer's recommendations.

We also compared data from manually prepared 96-well reactions to semi-automated robot-prepared 384-well reaction plates and obtained comparable results (not shown).

Primers were purchased from Eurogentec (Maastricht, Netherlands), and qPCR was performed in MicroAmp Optical 96-well Reaction Plates (Applied Biosystems) on a StepOnePlus Real-Time PCR system (Applied Biosystems).

SybrGreen qPCR reactions were performed in MicroAmp optical 384-well reaction plates, (Applied Biosystems).

Real-time PCR was performed using PCR Universal Master Mix Applied Biosystemss) in a MicroAmp 96-well reaction plate.