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The confocal microscopy observations and subsequent computer-assisted image analysis (Olympus Microimage™ Image Analysis, software version 4.0 for Windows, USA) revealed that Lipofectamine RNAiMAX at the concentration of 0.8 μl per 100 000 cells per well proved a highly effective transfection reagent ensuring the transfection efficiency at the level of 90%.
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"Well, prove a point to who?
This may well prove a disastrous move.
A mix of the two might well prove a very happy medium.
As the tournament progresses he may well prove a liability whom Portugal cannot adequately protect.
If Interscope escapes unscathed in the federal probe, Suge Knight's undoing could well prove a boon.
It may well prove a broadly positive force, just as it has on other businesses.
Blair says he knew before leaving office that Brown could well prove a "disaster" as prime minister.
The I.B.M. bid could well prove a landmark in challenging that assumption -- or, as some are warning, bearing it out.
Keeping the air in the Ukip bubble could well prove a more formidable challenge in the next vital six months.
Even with such talent on offer, the contest itself may well prove a somewhat dull, obscure affair.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com