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After 7 days, 75 µL supernatant was removed from each duplicate well, pooled, and kept at −70°C until IFN-γ concentrations were determined using a NHP IFN-γ IFN-γ ELISACell Sciences, Canton, USA).
After 7 days, 75 μl of cell culture supernatant was removed from each duplicate well, pooled, and stored at −80°C until IFN-γ and IL-17 were determined by ELISA.
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150 μl of supernatant was harvested from each of the three antigen/control wells, pooled in a micro centrifuge tube (total of 450 μl per antigen per study participant), divided into 3 aliquots and frozen at -80°C until further use.
Exposures were grouped into country specific as well as pooled tertiles as well as being used as continuous variables for statistical analyses.
In brief, the entire BAC library was plated (977 plates; approximately 1,200 colonies per plate) and colonies were transferred into ten 96-well plates with approximately 1,000 BAC clones per well (pool).
Cells were collected in 350 uL of QIAzol Lysis Reagent (Qiagen) per well and like wells were pooled.
We computed the number of negative droplets as if all droplets from three replicate wells were pooled together into one well.
Several case-control and cohort studies, as well as pooled data analyses, indicate that migraine is a risk factor for stroke and other vascular events [1 6].
The RTLU was compared to chromosome arm size as measured in Mb for individual subjects as well as pooled data.
Values from both regions of each well were pooled and averaged across all 6 replicates.
Increasing HRM sensitivity would improve mutation analysis of heterogeneous tissue samples, as well as pooled individuals.
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