Sowing and raising seedlings were conducted in a 96-well polymerase chain reaction (PCR) plate with a hole (Φ = 2.5 mm) at the bottom; one seed was placed in each well with the embryo pointing upward, and seedlings at the 3 ~ 4 leaf stage were transplanted to a paddy field.
DNA samples were distributed into 96-well polymerase chain reaction (PCR) plates and dried.
Memory and plasmablast (PB) B-cell subsets were sorted into single wells of 96-well polymerase chain reaction (PCR) plates, and rearranged immunoglobulin heavy-chain (IgH) genes were cloned and sequenced as described previously [ 19].
Reactions, setup in 96-well polymerase-chain-reaction plates, were incubated at 30°C for 1 hour.
Kits for expression, DNA isolation and purification, as well as polymerase enzyme and Ni NTA resin were obtained from Qiagen (Valencia, CA, USA).
An excellent example here is the well established polymerase chain reaction (PCR).
Computational as well as polymerase chain reaction (PCR -based aPCR -basedwere employed to further investigapproacheslative periods of activity for each family of elements (fig. 2).
The diagnosis of meningitis was based on clinical symptoms and cerebrospinal fluid examinations and cultures for bacteria as well as polymerase chain reaction (PCR) for viruses.
On the basis of clinical suspicion, additional diagnostic tests were added, such as culture for filamentous fungi, Mycobacteria spp. and Legionella spp., as well as polymerase chain reactions for the detection of Chlamydophyla pneumoniae, Mycoplasma pneumoniae and viruses.
In the last couple of years ChIP-Seq has become the method of choice for the genome-wide characterization of TF binding, as well as polymerase binding and histone modifications.
Both P2 as well as polymerase sequence analysis demonstrated the emergence of a recombinant strain without novel amino acid substitutions in the P2 region, as has been reported for emerging GII.4 variants (9 ).
