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Though the leak had slowed before it was successfully capped, a government-commissioned panel of scientists estimated that 4.9 million bbl had already leaked from the well, of which about 800,000 bbl had been captured.
For this analysis it was assumed that 4 million gallons of water are used per well, of which 50% flows back and is sent for treatment.
79 primer pairs were taken from Hanley et al. (2006) and tested in the parents as well, of which 70 resulted in clean PCR products.
Primers and probes were mixed with TaqMan® Universal PCR Master Mix, No AmpErase® UNG (Applied Biosystems, Foster City, CA, USA) and added to 96-well microtitre plates, each well of which contained 10 ng of air-dried DNA.
Expression of only 30 genes (20%) is significantly (P < 0.05) regulated in the TDG P65A/CBP condition as well, of which notably expression of 19 genes is regulated in the opposite manner, when compared to the wild-type TDG/CBP condition.
192 separate M13 primers labelled with a unique six bp 'barcode', the sequence of which could be used to identify the pool from which a product had originated,were used to allow two pooled plates, each well of which had a uniquely tagged product, to be combined and run per Illumina lane.
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Driving through Oklahoma's countryside, you see starlings and cows and nodding donkeys — also known as pumpjacks — and hundreds of disposal wells, of which there are around thirty-two hundred in the state.
There are 1,640 samples from nine cored wells, of which the porosities and permeabilities were tested in the laboratory.
Table 1 Coordinates, completion date and total depth of wells, which were used for T-logging and wells of which T-logs were provided by the MRMWR (NB-1, NB-22, NB-19) ID Coord.
After a 90 minute recovery time, 100 μl cell suspension was placed in each well of a 96-well microtiter plate (Iwaki), some wells of which were precoated with fibronectin or BSA.
The water source for those purposes is mostly from the rainwater, from the drilled and self-dug wells of which the water is alum poisoning or salt infiltrating, and from the spring water that is not well-treated.
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