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They are elegant portraits, as well, of two different ways of being a man.
This is your job?" It proved to be the job as well of two administrations (and now, it seems, a third).
The cell suspension was thoroughly mixed and 20 μl was pipetted into each well of two 60-well plates per replicate (Nunc Minitrays catalog number: 163118, Nunc A/S, Roskilde, Denmark).
Primary fibroblasts, 2 × 10, HDFn (hNaa10-WT) and one Ogden boy (S37P; III-6), at Passage 4 were plated in each well of two 12-well plates, and left overnight to adhere.
Adaptor 1*: 5′AATGATACGGCGACCACCGAGATCTACACGCCTCCCTCGCGCCATCAG-3′; Primer 1*: 5′-AATGATACGGCGACCACCGA-3′; Primer 2*: 5′-CAAGCAGAAGACGGCATACGA-3′; Adaptor 2: 5′-CAAGCAGAAGACGGCATACGAGAT-[BAR CODE]*-CGGTCTGCCTTGCCAGCCCGCTCAG-3′ Adapter 2 carried a 7 bp barcode sequence unique for each well of two 96-well plates (Supplementary file 2I).
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"THE Irish are a fair people," Dr Johnson once said, "they never speak well of one another".
Two × 10 cells were plated into each well of six well tissue culture plates.
For transfection, 2 × 10 cells were placed into each well of six well plates for 12 hours.
Migrated cells were counted using a light microscope at 160× magnification in three random fields per well of three separate wells.
A 1 cm piece of filter paper was added to each well of six 24-well plates, as a substrate for desiccating animals to attach to [ 50].
The single cell suspension derived from two 2-mths-old C57BL/6 was plated on 9 wells of two 6-well plate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com