Exact(3)
Internalization studies: The culture medium was removed, and the cells were washed once with 500 μL DMEM-F12 (5% BSA) before being left to equilibrate in 200 μL DMEM-F12 (5% BSA) at 37°C for a minimum of 15 min. Then, 25 μL (per well) of either DMEM-F12 (5% BSA) or of a 100-μM PMPA solution (blocking) was added, followed by the addition of 25 μL of 68Ga/177Lu-labelled PSMA ligand.
Briefly, the 96-well microplates were coated with 0.5 µg per well of either purified recombinant Omp3 or Omp7, or crude E. coli protein in coating buffer (0.1 M sodium bicarbonate, p.H 8.6) and incubated overnight at 4°C.
The plate was incubated for 1 h at 37 °C with shaking, washed and incubated with 100 μl per well of either biotinylated goat anti-human VEGF (0.1 μg ml−1; R&D Systems) or mouse anti-human VEGF165b (0.25 μg ml−1) for a further 1 h at 37 °C.
Similar(55)
For the first 10 passages the cells seeded in coated wells of either equivalent or double surface in order to achieve a two fold dilution.
Cells were plated into individual wells of either 96- or 6-well culture plates and grown in supplemented medium until confluent.
Kimberly Wong, 17, said she had an economics final coming up and was worried that retaking the calculus test would make it hard to do well on either of them.
That Mattingly essentially is admitting he cannot get Ethier to play hard every day does not reflect well on either of them.
Leave a couple of wells on either sides of the gel empty.
Anoikis resistance assays were performed by seeding 1 × 10 cells into the wells of six-well plates of either normal tissue culture grade (TCP; no. 140675, Nunc, Denmark) or ultra-low attachment grade (ULA; no. 3471, Corning).
DMSO only (0.5% v/v) wells, which contained no small molecule, were used as a negative control, while positive controls well consisted of either an FQ WT RNA only or WT RNA as a "small molecule" control.
It fell well short of either as it was dismissed for 93, giving Pakistan victory by 82 runs.
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