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This will have to be well introduced into the lateral cortex of the femur and properly tapped, thanks to the threaded part of the implant.
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These vectors (1 μg per well) were introduced into the cells using Effectene Transfection Reagent (Qiagen, Valencia, CA, USA).
This may well introduce considerable bias into comparisons between the two trial arms for end-points other than mortality and in such cases meaningful analyses can only be carried out for cardiac mortality.
"My analysis of Hillary Clinton is that there's almost this sense of entitlement, as if this country now has its own hereditary principle; you might as well introduce the House of Lords into Washington.
He fits into the village well, introducing himself as an écrivain who is working on a novel called Octet.
The cells were introduced into wells of a 24-well plate at a density of 1 × 10 cells/cm and incubated for 24 hr.
During the following years, several new mechanical ventilation modes and therapeutic measures, as well as ECMO, were introduced into the therapy for ARDS.
In brief, nuclear extract (5.0 mg of protein) from cultured cells was introduced into each well of 96-well plates coated with oligonucleotide containing the NF- κB consensus site (5′-GGGACTTTCC-3′) in a volume of 20.0 μL, followed by incubation for 1 h at 25°C.
A total of 5 × 10 target cells were introduced into each well of a two-well chamber slide (Lab-Tek), in which a 1 × 2 cm Ad-SiNWS was placed in the bottom of the chamber.
In total, 200 μl of a cell suspension with a cell density of 1×10 per ml was introduced into each well of a 96-well plate for 24 h.
One hundred microliters of each concentration was introduced into a well (96-wells microplate) containing 90 µl of SDB or MHB and 10 µl of inoculum was added to obtain a final concentration range of 256 0.125 µg/ml.
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