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Co-culture experiments were performed in transwell plates with 24 well inserts with 5 µm pore size (Corning Incorporated).
The AlvetexScaffold polyHIPE and associated plastic clips and well inserts used to house the polyHIPE membranes during cell-culture were supplied by Reinnervate.
Gels seeded with single-cell suspensions at a density of 5×10 cells ml−1 were prepared in sterile well inserts (1-inch outside diameter, Collaborative Biomedical Products, Bedford, MA, USA) with porous, 1 μm filter membranes.
Brown Centrex columns, Plate Crystalbridge well inserts, and ComboPlate 24-well polystyrene protein crystallization plates with lids for sitting-drop vapor diffusion were purchased from VWR. 5-EU phosphoramidite was purchased from Berry & Associates.
Cells were suspended in 3 ml collagen or a 3 ml Matrigel™-collagen mixture and seeded into 35 mm well inserts of a six-well plate (Organogenesis, Canton, MA) as previously described [ 32].
Briefly, UC-MSCs (150,000), which were fixed with 1% paraformaldehyde, were distributed to wells with FLSs (150,000), or UC-MSCs (150,000) were added in the lower well of the invasion plate, with FLSs (150,000) alone in the well inserts.
Similar(54)
Fine mesh chains appear as a signature as well, inserted like a label inside the waistband of jeans or the collar of a jacket.
And as for Shearer, if he had been as bad at scoring goals as he is at analysing them … well, insert your own Torres gag here.
Going hungry for a while en route, the article suggests, is the key to remaining alert for the transition to a new time zone — and considering the state of airline food these days, well, insert your own punchline here.
This meant that Fe components were well inserted into the framework of the TiO2 anatase structure.
The honeycomb MnO2 nanospheres are well inserted and dispersed on the graphene.
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