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The following sequence was grown on an SI GaAs (001) substrate: 40-nm undoped Al0.33Ga0.67As layer, 20-nm GaAs quantum well inserted with 2.15 monolayer of InAs quantum dots in the center, a 40-nm undoped Al0.33Ga0.67As spacer, a 20-nm Si-doped Al0.33Ga0.67As, and finally a 10-nm GaAs cap layer.
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Bioengineered cementum-like tissue and PDL-like tissue with well inserted Sharpey's fibers were demonstrated via cells, biomaterials, and growth factors.
For silver staining experiments, Ana-1 cells were seeded at a density of 2 × 106 per well in 6-well plates inserted with polylysine-coated cover slips for 24 h and treated with fresh medium containing PEGylated Au DENPs (1 and 3 μM) for 24 h at 37°C.
Co-culture experiments were performed in transwell plates with 24 well inserts with 5 µm pore size (Corning Incorporated).
Effect of CPP ecp on cell migration was assessed using a 24-well transwell plate inserted with incorporating polyethylene terephthalate filter membrane with 8 μm pores (BD FalconTM Cell Culture Insert System).
Approximately two weeks post-infection, cells were serum-starved overnight and 1×105 cells were seeded the next day in triplicate into BD BioCoat control trans-well inserts with 8-µm porous polyethelene terepththalate (PET) membranes (for migration assays) or Matrigel-coated invasion chambers (BD Biosciences).
Cell migration was assessed using 24-well inserts with 8 μm pores as described before (Wu et al, 2013).
Trans-well inserts with 8 µm filters (Greiner Bio-One, VWR, Radnor, Pennsylvania, USA) coated with 10% MatrigelTM (BD Biosciences) were used as a barrier for invasion.
Cell invasion was determined by a modified microchemotaxis assay [ 20] using cell culture 24-well inserts with the bottom sealed by a 8 μm pore polycarbonate-filter coated with Matrigel (Chemicon International, Inc .. Briefly, 2.5 × 105 cells in 250 μl of serum-free MEM were seeded into the inserts.
The active region of our proposed detector structure consists of a quantum well as in QWIPs, but inserted with submonolayers (SMLs) of a lattice mismatched semiconductor.
For 3-D experiments: SMCs and MFBs were suspended in rat tail collagen I (BD Science) gels and plated in 6-well cell culture inserts with 8 µm pore size (cell density: 2.5×105 cells/ml; gel volume: 1 ml; final gel concentration: 4 mg/ml); cells were then cultured for 24 h with 2 ml growth medium in the bottom well [19].
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