Sentence examples for well incubated from inspiring English sources

The original version carried a quote saying Hughes had been well incubated.

Subsequently, MTT reagent (150 μL, 0.8 mg/mL) was added in each well, incubated for 5 h.

After washing with phosphate-buffered saline (PBS), a horseradish peroxidase-conjugated cytokine or growth factor-specific antibody was added to each well, incubated for 2 h, and washed.

Sperm suspension (2 ml) was added to 500 μl of AgNP-treated eggs in sea water, mixed well, incubated, and observed for fertilization and developmental stages until the control attained 2 3-cell stages.

Then, aliquots of 5 μL treated or untreated supernatant were correspondingly added into two new plates with 95 μL 5.0 mg mL−1 xylan in each well, incubated at 50 °C for 10 min and stopped by adding 50 μL DNS reagent.

The screens are relatively simple with 5 larvae per well incubated from 1 5 days post-fertilisation with an experimental drug.

50 µl of activated XTT solution was added to each 96 well (1×104 cells/well), incubated at 37°C for 4 hours and absorbance was read at a wavelength of 450 nm.

For 75Se-labeling, cells were seeded onto a 6 well plate (3×105 cells/well), incubated 24 hours, then labeled with 40 µCi of 75Se for 24 hours, harvested and lysed as described above.

Cells were seeded into 6-well plates (0.5 × 10 cells/well), incubated overnight, treated with the indicated concentrations of CGE, and then incubated again for 48 h.

The HUVECs were seeded in 6-well plates (1 × 10 cells/well), incubated in M199 with 10% FBS for 24 h, and subsequently washed twice with PBS and incubated in M199 with 1% FBS.

For promoter activity assays, 2BS cells were seeded in a 24-well plate (1 × 10 cells/well), incubated overnight in complete growth medium, and transfected with 0.45 μg of reporter plasmid and 0.05 μg of control reporter (pRL-CMV).