Sentence examples for well ice cold from inspiring English sources

The coverslips were placed in 24 well plates, and PBS was removed from each well, ice cold 100% methanol was then added and immediately removed, then 200 µl YOYO working solution (1.5 µM) was placed over fixed cell populations for 30 min at 22 C in a darkroom.

Matrigel was thawed on ice and 100  μL solution was evenly added to each well of ice cold 24-well plate and was kept in incubator for 30 minutes.

He stipulates: " Three measurements of Gordon's, one of vodka, half-a-measure of Kina Lillet, shake it very well until it's ice cold, then add a large, thin slice of lemon peel.

The cells were fixed by adding 100  μl per well of ice-cold 40% TCA to each well for 60 min.

A six-well plate was layered with 0.775 mL (each well) of ice-cold collagen gel mixture consisting of 0.5 mL collagen stock, 0.15 mL 10× modified Eagle medium, and 0.125 mL 0.5 N NaOH.

After 24-h injury, wells were rinsed once in ice cold PBS, and incubated in 4% paraformaldehyde for 25 mins at 4 °C.

Subsequently the wells were washed 10× with ice cold PBS or 1 50 anti-p24 mAb in Ch-PBS for HIV-1 BaL to minimize the contribution of p24 antigen not associated with virus particles to absorbance readings corresponding to p24 antigen released from detergent treated virus.

Following treatment cells were washed with PBS and fixed with 100 ul ice cold methanol per well.

Five drops of concentrated H2SO4 were added to the ice cold solution, stirred well, and heated on a water bath (50°C) for 30 min.

The cell suspension was combined with 9 ml of ice cold PBS, mixed well by inverting the tube, and centrifuged at 1000 × g for 5 min at 4°C (recovery of cells from suspension was always accomplished by centrifugation under these conditions).

The cell pellet was resuspended with 35 μl/per well of ice-cold RPMI-1640 medium, and the cell suspension was kept on ice.