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About 25% of household respondents reported that their children are not well grown for their age.
Among children thought by their mothers to be well grown for their age, 35% were chronically malnourished and 5% were acutely malnourished.
Among children thought by their mothers to be well grown for their age, 42.5% were chronically malnourished and 14.5% were acutely malnourished.
Cells were plated at 5,000 cells per well, grown for 24 hr, and then treated with CBP (0 300 μM, Mayne Pharma, Victoria, Australia) for 72 hr in normal growth media.
Briefly, cells in exponential phase of growth were plated in six-well tissue culture plates at 1 × 10 cells per well, grown for 24 h and then transfected with siRNA using Oligofectamine and OPTI-MEM I-reduced serum medium.
Breast cancer cells were propagated into six-well plates at 2 × 10 cells per well, grown for 24 h in complete medium followed by transfection with ON-TARGET plus Smart pool siRNA specific to Puma, HRF and Mcl-1 (Dharmacon, Chicago, IL, USA) or scrambled siRNA-negative control at a final concentration of 20 n using lipofectamine reagent.
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Though as AI proliferates and touches more and more decisions, and as its impacts on people and society become ever more evident, pressure may well grow for proper regulatory oversight of algorithmic blackboxes.
Cells were seeded in 96-well plates at 8000 cells per well and grown for 18 h.
Cells were seeded in complete growth medium in 24-well plates (5 × 10 cell per well) and grown for 24 h.
Cells were seeded in standard 6 well plates at a density of 5×104 cells per well and grown for two days in culture medium.
H4 neuroglioma cells were seeded on 12 well plates at the density of 105 cells per well, and grown for 12 hours at 37C.
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