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Unlike classical tissue annotation and clinical data, these high-throughput techniques generate a large amount of well formatted data, typically for a relatively small number of tissue specimens.
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In addition, we have extended the use of the assay to function in a 384 well microtiter plate, which shared the same characteristics as the 96 well format (data not shown).
Again, independent of the membrane surface area, TEER values of Caco-2 monolayers measured with the CellZscope (24-well format) correlated to off-line TEER values measured manually with an EVOM using a 6-well format (data not shown).
However, a reduction to 86 features did not lead to a significant loss in predictivity, indicating that this set could be used for an assay in a 96-well format (data not shown).
True Open Notebook practitioners don't limit their open content to well-formatted data.
Without this streamlining, finding and retrieving pathways, downloading and re-formatting data as well as often having to perform ID mapping take considerable effort and time for a user.
Accomplishing this goal begins with the improvement of software tools for data entry and use of the most rigorously complete and well defined data formats, and culminates in changes to the culture of data publication.
The openness of source code as well as data formats and data itself allows better integration and interoperation between data resources.
The use of JSON, a standardized and well supported data format, to configure a visualization with GenomeD3Plot reduces the burden on developers and lowers the complexity of use.
The reaction was initiated at 50°C for 2 min and continued at 95°C for 10 min and followed by 50 cycles of 95°C for 15 s and 60°C for 1 min. The experiments were carried out on the 7900HT Sequence Detection System (Applied Biosystems) in a 384-well format, and the fluorescence data were collected by the "Absolute Quantification" application of SDS 2.3 software (Applied Biosystems).
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