The biofilm fixed on each well surface was extracted with 100 μL of 70% ethanol and measured the absorbance at OD570 nm.
The culture medium was removed after 72 h inoculation, the intracellular total RNA (in 96-well plates) was extracted with RNeasy Mini Kit (Qiagen), and total intracellular proteins (in 6-well plates) were extracted with Cyto-Buster Protein Extraction Reagent added with 1 mM protease inhibitor cocktail.
The culture medium was removed after 72 h inoculation, the intracellular total RNA (in 96-well plates) was extracted with RNeasy Mini Kit (Qiagen), and total intracellular proteins (in 6-well plates) were extracted with Cyto-Buster Protein Extraction Reagent added with 1 mmol/L protease inhibitor cocktail.
After 6 h, wells were individually extracted with 500 μL of EtOAc prior to chiral-phase GC analysis.
To rehabilitate a contaminated aquifer, contaminated groundwaters are withdrawn through drainage wells, PCBs are extracted with solvents or activated carbon and treated by chemical, photochemical or thermal methods.
At 48 hr, the ODS disks and the cells from both the BDS/Transwell exposure and BDS/no Transwell exposure wells were rinsed, extracted with DCM, and analyzed with GC/MS.
These strains were grown in 24 independent micro-cultures each in the wells of a 96 well plate, harvested and extracted with minor modifications to the procedure described in Figure 1 (a detailed description of the 96-well extraction is given in the Materials and Methods section).
The well-ground samples were extracted with ethanol for 12 h followed by repeated washings to remove extractives.
For the analysis of chlorophyll concentrations, 0.1 g of finely cut and well-mixed sample was extracted with 8 mL 95 % acetone for 24 h at 4 °C in darkness and shaken 3 or 4 times until they were bleached, according to the method of Tang et al. (2015).
The well-ground samples were extracted with ethanol:toluene (1 2) for 12 hours, followed by repeated washing with ethanol and water to remove extractives.
