Three fields in each well were examined by fluorescence microscopy by two individuals, and the mean ratio of the count of unstained cells to that of the total cell count was calculated as the cell viability.
The cell viability in each well was examined by a MTT (5 mg/ml, Sigma-Aldrich, Cat.M2003) colorimetric assay.
PCR products from each well were examined by electrophoresis, and products of the appropriate size were sequenced by a commercial facility (SeqWright, Houston, TX).
After 3 4 weeks, wells were examined by light microscopy for outgrowth of lymphocytes.
In the context of CO2 huff-n-puff EOR, the effect of fracture complexity on well productivity was examined by simulation approaches.
Control well images were examined by eye and the Acapella spot detection algorithm was used to obtain the optimum focus height for the greatest differentiation between the control wells.
The mixed cultures were grown to visible density at 30°C and then pipetted into a well of a glass-bottomed 96-well plate and examined by microscope.
The involvement of CpFas2 in biofilm formation on polysterene (96-well plate) and silicone sheet surfaces (24-well plate) was examined by a metabolic activity assay with XTT and by fluorescence microscopy.
Twenty-four hours later, the 96-well plates were examined by fluorescence microscopy.
