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A number of important building energy issues and efficiency features cannot be sufficiently well evaluated using any of the existing tools.
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The cytopathic effect observed in the virus-containing wells was evaluated using a microscope and the TCID50 was calculated using the Reed-Muench method.
Cell viability (12 × 103 cells per well in a 96-well plate) was evaluated using a colorimetric MTT assay measuring reduction power (Roche Diagnostics).
In order to estimate DNA degradation; the main DNA band per well was densitometrically evaluated using the Quantity One Program 4.2 [ 32].
34, 35 Psychological well-being was evaluated using the Philadelphia Geriatric Center Morale Scale (PGCMS) (scores range 0 17).
General well-being was evaluated using an adapted Smilie-test [ 43] and body image was evaluated using the Figure Rating Scale [ 44] during a 1 1 interview, performed by trained examiners.
The viability of the treated cells grown in 96-well plates was evaluated using MTT assay as described previously [ 17] and cell viability was expressed as the percentage of the control group.
Subjective well-being was evaluated using the Portuguese version of the Personal Well-being scale, which includes seven areas (satisfaction with level of life, health, personal achievement, personal relationships, sense of safety, community connection and future security).
S elf-care and well-being are evaluated using the Health-Promoting Lifestyle Profile II (HPLP-II), self-efficacy, self-rated health, class attendance, reports of continued practice yoga after completing the study intervention, as well as qualitative data (participant comments).
These activities were evaluated using well diffusion method.
The antibacterial activity of biosynthesized AgNps was evaluated using well diffusion technique.
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