This was achieved by using yeast strains carrying the HIS POL30 allele and a well established pull-down protocol [ 46].
But even when the artists are well established, Rego has managed to pull something slightly unusual out of the cupboard.
Although the existence of pulling forces is well established by laser-cutting experiments [7], [8], and genetic and RNAi experiments have provided insight into the molecules necessary for force generation [9], individual force generation events have not been observed in vivo.
Peptide competition for the native LapD LapG interaction was assessed using an established pull-down assay (Newell et al., 2011a).
Morningstar equity analyst Brett Horn, for one, believes that the impact of any legislation would be limited, as "the economic pull of wealthier countries to immigrants is a well established trend".
The existence of asymmetric microtubule-dependent pulling forces in the one-cell embryo is well established.
A major source of the pain is well established - Mr Obama's problematic relationship with Prime Minister Benjamin Netanyahu, who pulled out a hard-fought electoral victory and appears to have the inside track to form a new Israeli government.
Numerous biochemical and genetic approaches such as GST-pull-down assays, coimmunoprecipitation, yeast two-hybrid screens and protein microarrays are well established techniques for analyzing protein-protein interactions.
Most are well established.
The facts are well established.
