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This is well done by using an array of computational techniques interrogating both sequence and structure.
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This transformation is done by using the well-known node splitting technique that has proven useful for different network design problems, see e.g., [4,7].
was done by using agar well diffusion assay (Valgas et al. 2007).
Predicting the vibro-acoustic behaviour of a structure is usually done by using the well established finite element (FE) method and boundary element method.
The main purposes of green roofs in Poland are: to create additional green space in dense urban area and to increase biodiversity, which could be done by using native plants (well adapted to local weather conditions).
Multiple-sequence alignment was done by using Mac Vector, as well as ClustalW, while phylogenetic tree analysis was done by using Phylogeny.fr.fr
Amino acid alignment was done by using ClustalW2 as well as HMMER tools (http://hmmer.janelia.org, Finn et al. 2011), and the phyla and orders were predicted using an E value < e−20 as a cut-off.
The calculations of the indices of each PHC dimension as well as the global performance index were done by using procedures that took into account the complex nature of the sample design (complex sample module of SPSS 21.0 software) and controlled for possible clustering when calculating the variances of averages.
Spectrophotometric measurements were done by using a 96-well micro-plate ELISA reader (Infinite M200, Tecan, Switzerland).
ChIP assays were done by using protein A-coated 96-well polypropylene microplates as described before [ 27].
Well done by Mr. Chelsea.
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