Stir in the apples until well distributed, then spoon into the tin.
25 µl each of lysate from a single well was distributed into three wells and firefly and renilla luciferase activities were sequentially measured with L-Max luminometer which injected 100 µl each of LAR II buffer and Stop&Gro buffer (Molecular Devices, Sunnyvale, CA).
Stir the honey and oil into the bowl until well distributed.
It is well distributed but poorly marketed.
Precipitation is reasonably well distributed.
The remaining half of PBMCs were distributed into wells of 96-well plate of a final volume of 0.2 ml per well with RMPI 1640 enriched with 10% Fetal Bovine Serum (Biochrom), 2 mM glutamine and 10 mM pyruvate at a density of 2 × 106 PBMCs/ml.
Cell suspensions were then distributed into wells of a 96-well plate or 384-well plate, respectively, using an automated reagent dispenser and plates were incubated at 37°C.
Briefly, TS cells were sorted by fluorescence-activated cell sorting using the MoFlo system (Beckman Coulter, Brea, CA, USA), and individual cells were distributed into wells or 96-well plates containing 5 μl of CellsDirect resuspension buffer (Invitrogen, Carlsbad, CA, USA).
Briefly, TS and XEN cells were sorted by fluorescence-activated cell sorting using the MoFlo system (Beckman Coulter, Brea, CA, USA), and individual cells were distributed into wells or 96-well plates containing 5 μl of CellsDirect resuspension buffer (Invitrogen, Carlsbad, CA, USA).
The cells were then distributed into wells of a six-well plate at 60% confluency and allowed to adhere.
