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GI-1 cells were seeded in glass-based bottom well dish at a density of 1 × 104 cells/ml.
Each agarose ring was placed in one well from a six well dish.
Cells were plated at 4 × 10 cells per 6 well dish.
i. 1 day before harvesting viral supernatant, plate 1.2 × 10 HT1080 cells per well of a six well dish.
Bladder cell lines were seeded in a 6 well dish with a concentration of 3 × 10 cells/cm.
[H]-thymidine incorporation was assessed in triplicate of cells seeded at a density of 50 000 cells per well of a six well dish.
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Briefly, a 6-well dish of ESCs was scraped and lysed in TRIzol (RN190-200, Invitrogen).
The remained cells were diluted and plated in a 96-well dish to screen for positive monoclonal cells.
For the investigation of cytotoxicity of nanoparticles, 1,000 cells per well of 96-well dish were plated and allowed to adhere overnight.
The MC3T3-E1 cells were incubated for 3 days on scaffolds in a 4-well dish following the steps as used in von Kossa and Alizarin Red assays.
Cells were plated in a 12-well dish (5×104 cells/well).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com