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An aliquot was removed from each well diluted 1 10 and 1 100 dilution and 50 μl were plated on 7H10 ágar.
To quantify patient samples, one standard curve was generated by plotting the average cycle threshold (Ct) values for all integration signals at each Integration Standard (IS) dilution (70 0.2 copies of IS standard per well, diluted in 2 µg/ml PBMC DNA), so long as at least one integration signal was significantly different (two standard deviations) to the average gag-only background signal.
In order to evaluate the proportion between the number of larvae (L3) and the fluorescence signal, initial concentration of 2000 L3 larvae per well diluted in M9 medium was used for fluorimetric tests followed by 1 : 2 serial dilution of L3 larvae per well.
An ounce or two of whiskey well diluted with water, as in a conventional highball, is absorbed less rapidly than the same amount poured over ice.
Phages particles (1010) per well diluted in PBS-T were added and incubated for 1 h, then the unbound phages were removed by washing with PBS-T.
As in this experiment, the calcium carbonate solution was oversaturated and 1% TSB was well diluted, the influence of organic ligands can be ignored.
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A common practice is to hire local employees who know the geographic culture, even though this may well dilute the company culture.
At a confluence of approximately 75%, they were incubated with 0, 4, 8, and 10 μg galactosylated poly- l-lysine DNA compl-lysine DNAwell diluted in 2 mL medium, respecomplexes
The MBC was evaluated by pipette each dilution from the clear wells, diluting with PBS pH 7.2 and then 10 µl of each dilution was dropped onto Ashdown's agar for colony counts.
Briefly, same copy numbers per well of diluted VP1, VP2 and VP3 as well as the truncated VP2 or VP3 subunit proteins were used to coat a 96 wells plate for 1 h at 37°C.
After overnight incubation at 37°C, inoculums from each well were diluted 10-fold and plated on MHA plates to determine the CFU/mL of each well.
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