The subsequent sections describe these engineering components of automated DOF workflows: virtual multiphase metering, smart production surveillance, well-test validation, and well diagnostics.
Fibroblasts were plated at 70% confluence in 6-well plates for FACS analysis or for immunocytochemistry purposes on 12-well diagnostic microscope slides (Cel-Line, Thermo Scientific, Braunschweig, Germany) and allowed to adhere for at least 6 hours before cells were treated.
For immunofluorescence studies, 5 × 105 cells were seeded onto 3-well diagnostic microscope slides (Erie Scientific Company) in 10 cm cell culture dishes.
Briefly, M. xanthus cells were grown as described and fixed with 2.6% paraformaldehyde and 0.008% glutaraldehyde for 20 min on freshly prepared poly- L-lysine-treated 12-well diagnostic slides (Erie Scientific Company).
Those procedures included the implantation of stents to open clogged arteries as well as diagnostic tests.
We have also introduced customized placement tests aligned to individual state academic standards, as well as diagnostic and online intervention tools for students.
This further step can help at reaching robust clinical evidence as well as diagnostic consensus.
Despite well established diagnostic criteria for acute kidney injury (AKI), the detection is often delayed in the clinical practice.
