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Although all parameters identified for the decision algorithms have been used as diagnostic markers of EOS earlier [ 25] this analysis allowed to identify cut-off values for AB treatment failure, as determined by the data, at well defined time points.
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Full as well as sole observational scoring was performed at defined time points during an evaluation period of 30 h.
The advantages of studying infectious disease in rhesus macaques include the ability to manipulate the dose, route, and strain of the infectious agent, as well as the ability to analyze specimens from defined time points.
To measure the effect of sera on cellular growth, total protein was measured at defined time points during culture in 6-well plates by the colorimetric method [ 37].
Such guidelines might include directives of a priori defined time points, or quantiles of survival-time of interest, as well as determining what effect should be regarded as clinically significant at these time points or quantiles.
At defined time points, 100 μl of the cell suspension in each well was quenched with 200 μl acetonitrile containing an internal standard.
Hemorrhaged and resuscitated animals were sacrificed on defined time points (immediately after resuscitation [t = 0 hours] as well as at t = 24 hours and t = 72 hours thereafter) by cervical decapitation.
5 × 103 cells were plated in 96-well plates, incubated for 24 hours at 37°C, and treated with specified agents at defined time points.
At defined time points (after 0, 1, 3, 6, 12, 18, 21, 26, 29 months) the steel cages were dug out and the gauze bags as well as the surrounding soil were taken for sampling and analyzing.
Cells were subsequently fixed in paraformaldeyde at defined time points and subsequently stained with cristal violet.
Fluorescence recovery was followed by photographing animals at defined time points.
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