Similar(59)
The experiment was conducted using both basal DMEM with no additives as well as defined medium without B104 (described in the Methods) with similar results.
In this assay, OPCs were seeded on PDL-coated 96-well plate in defined medium plus 1% horse serum at a cell density of 4×10 cells/well.
Three serum-free media, three protein-free (animal component free) media, as well as one chemically defined medium were included in the study.
These three peptides were found to support neural stem cell self-renewal in defined medium as well as multi-lineage differentiation.
The growth and fermentation performance was evaluated in defined medium as well as in a complex medium containing glucose and xylose.
CeMM can be used in a solid or liquid state, it can be stored unused for at least a year, unattended actively growing cultures may be maintained longer than with standard techniques, and standard C. elegans protocols work well with animals grown in defined medium.
To systematically study the intergenic transcriptome of R. etli under diverse conditions, we opted to determine transcription profiles at various time points during free-living growth in defined medium as well as during the nitrogen-fixing endosymbiosis with its eukaryotic host plant P. vulgaris, totaling 5 sampling conditions.
Differentiation was initiated by allowing EBs to form in cultures of disaggregated ESCs seeded at 1×104 cells/ml in non-adherent 6 cm bacteriological dishes (Phoenix Biomedical) or by spin EB formation with 5×102 cells seeded per well in non-adherent round bottomed 96 well plates (Nunc) in a chemically defined medium (CDM) [17], [23] as described [61].
SCG neurons were cultured at very low density on poly-ornithine- and laminin-coated 4-well tissue culture dishes in defined medium.
The feasibility of the clinical development of hESC-based therapy has been improved by the scalability of hESCs as well as the formulation of chemically defined medium containing material only from human ('xeno-free' medium) for hESC culture (Table 1).
The upper chambers were inserted into the tissue-culture wells and 750 uL culture medium containing 1% serum, defined medium (consisted of DMEM/F-12 supplement with 1% N2 (vol/vol), 10.1 ng/mL T3, 400 ng/mL T4, 0.035% bovine serum albumin (BSA) and 20 µM Leupeptin) or condition-defined medium were added to lower chamber.
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