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When colonies had reached sufficient size (at least 25%% well confluence), data from the Cell Metric™ was used to generate a pick list for each cell line.
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To estimate the total number of spots in each well with confluence, the following equation was used: total spot number = spot count + 2 × (spot count × % confluence / [100% - % confluence]).
The fishermen before me were waiting their turn to jog down the well-worn path on the bridge's west abutment so they could gain access to the well-publicized confluence.
Once the wells reached confluence, the Culture-Inserts were removed creating a 1-mm gap (wound).
Like my earlier book, Sacred Waters: A Pilgrimage to the Many Sources of the Ganga, it explores the mysteries that lie beyond each destination as well as the confluence of natural history, myth and Himalayan lore.
Cells were cultured in 6 well plates to confluence and Hoechst 33342 (Sigma, USA) was then added.
Cells were cultured in chamber slides (Lab-Tek) until they reached a density of 2 × 10 cells per well (≈ 90% confluence), at 37°C in 5% CO2.
HCA-7, HT-29, HCT-116, NCM-460, HeLa and HEK-293 cells were seeded in 6-well plates (∼7 × 10 cells per well) at 70% confluence.
The cultures considered here ceased proliferating well before 80% confluence and displayed disorganized cell geometry and cells often detached from the permeable support.
BEAS-2B CYP2S1 depleted cells (759 and 984) and scrambled controls (SCRAM) cells were grown in a six well plates to confluence and washed with PBS.
After reaching 50% confluence, wells were treated with the indicated concentration of butyrate for 24 hrs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com