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Cells were seeded into 6-well plates at a density of 1 × 105 cells (alkaline phosphatase assay, PCR analysis) or into 96-well plates at a density of 5 × 104 cells per well (cell viability assay).
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These biological tests showed that GE/PLGA-AgSD scaffolds had good cell viability, as well as effective antimicrobial activity.
Cells were plated at a density of 6 × 10 cells per 10 cm dish or 2.5 × 10 cells per well on 24-well plates (cell viability only).
For the cell proliferation assay, cells were seeded into 96-well plates at 3 × 10 cells per well and cell viability was measured at 24, 48, 72 and 96 hr after sorting using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan).
Their physicochemical properties, as well as cell viability, were assessed.
The cell uptake behavior as well as cell viability of PTH-CHO-PEI-DEP FNPs was examined to evaluate their potential for biomedical application.
The in vitro bioactivity in SBF and adsorption of bovine serum albumin as well as cell viability for all the prepared scaffolds were also tested.
We investigated the pore size and structure, porosity, swelling as well as cell viability of a series of recombinant human collagen-peptide chitosan (RHcollagen-peptide chitosanby lyophilization.
The results showed that most viable cells (green) survived with few dead cells (red), indicating that the GFs are capable of supporting the attachment of hMSCs, as well as cell viability.
The thermal and mechanical properties of both scaffolds were investigated and human mesenchymal stem cells were seeded onto the scaffolds to evaluate the cell attachment properties, as well as cell viability and differentiation.
Furthermore, the general expression of T cell signaling proteins and tyrosine phosphorylation rates as well as cell viability are unaffected.
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