Colour change in each well was monitored visually.
Next day, the fluorescence of each well was monitored using an excitation at 375 nm and emission of 445 nm with a BioTek Cytation 3 plate reader (Thermo Fisher Scientific Inc).
Each well was monitored for cell growth and confluency.
Plate reading started immediately, and each well was monitored automatically by the luminometer for 1 sec every minute for 1 hr.
The fluorescence changes from each well were monitored using an ABI PRISM® 7500 Sequence Detection System (Perkin Elmer, Inc, Waltham, MA).
Each well was monitored by an inverted microscope IM (Olympus, Tokyo).
Each well was monitored with an inverted microscope (IM; Olympus, Tokyo, Japan).
The fraction of live animals per well was monitored until 98.1% was no longer alive (59 783 of 60 921 animals).
Viable cells in each well were monitored by measuring fluorescence at 590 nm (with excitation at 535 nm).
After incubation, fungal growth in each well was monitored by observing and comparing the turbidity of the test wells to that of the positive and negative controls.
In all, 50 μg of protein was added, and then the OD of each culture well was monitored using a microplate reader at 550 nm for 120 min at 30 °C.
