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One well was loaded with 4 μl of HiMark™ Pre-Stained Protein Standard (Novex®).
Irrespective of protein concentrations, each well was loaded with equal volume (20 µL) of sample.
Depth of the well is loaded positively, as observed earlier for total chromium.
Agar wells of 5-mm diameter were prepared with the help of a sterilized stainless steel cork borer labeled as A and B. "A" well was loaded with 30 μL of colloidal AgNPs suspended "hydrosols", and "B" well was loaded with 30 μL of positive control drugs (ciprofloxacin) used as a positive controls.
Fifty micrograms of proteins per well were loaded.
Each well was loaded with 25 µl from each fraction.
Each well was loaded with 30 µg protein, separated by 10% SDS-PAGE (Nu-PAGE), and transferred to a polyvinylidene fluoride (PVDF) membrane.
For each tissue homogenate or plasma sample, 20 µg of total protein per well were loaded onto a 10% SDS-PAGE gel.
Total cell protein (OD600 = 0.01 corresponding to equal protein per well) was loaded onto a SDS-polyacrylamide gel (10% polyacrylamide, 0.1% SDS) [12], [18], [35].
Each well was loaded with approximately equal amounts of microcarriers.
Each well was loaded with 20 μg of proteins.
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