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It knows its clientele well, being grown-up enough to have clean, smart rooms which are en-suite – and a licensed bar and restaurant – but avoiding spending on unnecessary frills to help keep costs down.
Then, a co-culture of tumor spheres with 2 × 105 CART-HER2 cells or NT T cells per well, was grown in an ultra-low adherent 24-well plate using serum-free media.
Two hundred thousand and one hundred thousand HeLa cells per well were grown in 12-well plates, or 24-well plates, respectively in complete medium.
Cells (2000 per well) were grown in 96-well plates in 100 μl media per well.
MEFs (200,000 per well) were grown in 0.1% gelatin-coated 6-well plates for 24 h.
Cells (10 cells per well) were grown in 24-well plates and exposed to increasing doses of the drugs.
The cells at a density of 3 × 10 per well were grown onto cover slips in 6-well plates.
Briefly, cells (5 × 10 per well) were grown in 10% FBS medium in 6-well plates overnight for attachment.
Before TEER measurement, Caco-2 cells on each well were grown for 21 days and formed a polarized monolayer.
Cells (10 cells per well) were grown in 24-well plates and exposed to increasing doses of NVP-LDE225, everolimus, and sunitinib, alone or in combination.
For total cell death assays, 5 × 10 cells per well were grown in 96-well plate in serum-poor medium and treated with Doxorubicin.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com