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Detailed analysis of the signature model revealed that p53 is the dominating predictor and that the risk for an individual patient can be equally well assessed by using p53 staining status alone as by using the entire signature (Fig 6D).
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Psychological well-being was assessed by using the following four constructs: perceived stress, vital exhaustion, positive affect, and negative affect.
Perceived well-being was assessed by using a Con-Dis device and by filling out an attached questionnaire - RAI - at the same time.
Where data are presented as heat maps, the 2- CTgene-CT18S) (2- CTgene-CT18S as an approximate measure of expression to allow comparison of expression levels between genes because it has been shown to correlate well between copy number (as assessed by using in vitro-transcribed RNA to produce a standard curve) and cycle threshold (CT) values [ 30].
Perceived well-being has earlier been assessed by using RAI and RAVA [ 11- 14].
The study would have been strengthened if concurrent validity had been assessed by using a well-validated scale.
The role of LOXL2 in 3D development of normal breast epithelial cells was assessed by using the well-characterized MCF10A acini assay [ 14], in which cells are cultured on top of a layer of recombinant basement membrane (Matrigel) within a Matrigel suspension.
Alcohol dependence and alcohol abuse as well as mood and anxiety disorders were assessed by using a diagnostic mental health interview, Munich-Composite International Diagnostic Interview (M-CIDI), yielding the diagnosis based on DSM-IV criteria (Diagnostic and Statistical Manual of Mental Disorders, 4th edn, American Psychiatric Association 2000).
The effects of MSU crystals and HDL on the chemotaxis of mononuclear cells were assessed by using a 48-well modified Boyden chamber (AC48; NeuroProbe, Bethesda, MD, USA).
Renal function will be assessed by using estimation equations (MDRD and cystatin C) as well as measured creatinine clearance determined by 24-hour urine collection.
Renal function will be assessed by using estimation equations (Cockcroft-Gault and cystatin C) as well as measured creatinine clearance determined by 24-hour urine collection.
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